How much control does the gene gun process give you over where the new sequence ends up? Is it in the same place in all of your production seeds or do they structurally differ? I guess a related question is if all the seeds come from one ancestor cell where the insertion was done or if you do the insertion multiple times?
(I work with human genetics and am fascinated by the prevalence of structural variation.)
The gene gun inserts randomly. We do a large number of insertions and will then test to see which are the best plants and take those forward to production. All the seeds shipped will however come from a single parent.
(I work with human genetics and am fascinated by the prevalence of structural variation.)